Fiber-Optic Chemical Sensors and Biosensors (2015–2019)

 Xu-dong Wang*, Otto S. Wolfbeis

Analytical Chemistry 2020, 92, 397-430.

DOI:10.1021/acs.analchem.9b04708.

44. Luminescent Silica Nanosensors for Lifetime Based Imaging of Intracellular Oxygen with Millisecond Time Resolution

Longjiang Ding, Wei Zhang, Yinglu Zhang, Zhenzhen Lin,  and Xu-dong Wang*

Analytical Chemistry 2019, 91, 15625−15633.

DOI: 10.1021/acs.analchem.9b03726.

43. Quadruply-labeled serum albumin as a biodegradable nanosensor for simultaneous fluorescence imaging of intracellular pH values, oxygen and temperature

Xiao-ai Zhang, Wei Zhang, Qi Wang, Junli Wang, Guodong Ren, Xu-dong Wang*

Microchim Acta, 2019, 186, 584.

DOI：10.1007/s00604-019-3674-4.

42. Highly sensitive dissolved oxygen sensor with sustainable anti-fouling, anti-abrasion and self-cleaning superhydrophobic surface

Yinglu Zhang^†, Liang Chen^†, Zhenzhen Lin, Longjiang Ding, Xufeng Zhang, Ruihua Dai, Qiang Yan, and Xu-dong Wang*

ACS Omega 2019, 4, 1715−1721

41. A lysosome-targeting nanosensor for simultaneous fluorometric imaging of intracellular pH values and temperature

Wei Zhang, Y. G. Abou El-Reash, Longjiang Ding, Zhenzhen Lin, Ying Lian, Bo Song, Jingli Yuan, Xu-dong Wang*

Microchim Acta, 2018, 185, 533.

DOI：10.1007/s00604-018-3040-y.

40. A background-subtraction strategy leads to ratiometric sensing of oxygen without recalibration

Yinglu Zhang,Longjiang Ding, Wei Zhang, Xiao-ai Zhang, Ying Lian, Xu-dong Wang*

Analyst, 2018,143, 5120-5126.

DOI：10.1039/c8an00967h.

39. Fully-reversible optical sensor for hydrogen peroxide with fast response

Longjiang Ding, Siyu Chen, Wei Zhang, Yinglu Zhang, Xu-dong Wang*

Analytical Chemistry 2018, 90, 7544-7551.

DOI：10.1021/acs.analchem.8b01159.

38. Synthesis of highly stable cyanine-dye-doped silica nanoparticle for biological applications

Ying Lian, Long-Jiang Ding, Wei Zhang, Xiao-ai Zhang, Ying-Lu Zhang, Zhen-zhen Lin and Xu-dong Wang*
Methods and applications in Fluorescence, 2018, 6, 034002
DOI: 10.1088/2050-6120/aab930

37. Fluorescent proteins as efficient tools for evaluating the surface

Wei Zhang, Minyan Ma, Xiao-ai Zhang, Ze-yu Zhang,Sayed M Saleh and Xu-dong Wang*

Methods and applications in Fluorescence  2017, 5, 024003.

DOI: 10.1088/2050-6120/aa64e5

36. Two-Photon Excitation Temperature Nanosensors Based on a Conjugated Fluorescent Polymer Doped with a Europium Probe

Xu-dong Wang*, Robert J. Meier, Michael Schäferling, Sebastian Bange, John M. Lupton, Michaela Sperber, Joachim Wegener, Vladimir Ondrus, Uwe Beifuss, Ulrich Henne, Christian Klein, Otto S. Wolfbeis*. 

Advanced Optical Materials. 2016, 4, 1854-1859.

DOI: 10.1002/adom.201600601

35. Fiber-Optic Chemical Sensors and Biosensors (2013−2015)

Xu-dong Wang*, Otto S. Wolfbeis*.

Analytical Chemistry. 2016, 88, 203-227.

DOI: 10.1021/acs.analchem.5b04298

34. Multifunctional Silica Nanoparticles for Covalent Immobilization of Highly Sensitive Proteins

Xu-Dong Wang, Kersten S. Rabe, Ishtiaq Ahmed, and Christof M.Niemeyer*. 

Advanced Materials. 2015, 27, 7945-7950.

DOI: 10.1002/adma.201503935

33. A colorimetric assay for measuring iodide using Au@Ag core-shell nanoparticles coupled with Cu2+

Jingbin Zeng*, Yingying Cao, Chun-Hua Lu, Xu-dong Wang, Qianru Wang, Cong-ying Wen, Jian-Bo Qu, Cunguang Yuan, Zi-feng Yan*, Xi Chen. 

Analytica Chimica Acta. 2015, 891, 269-276.

DOI: 10.1016/j.aca.2015.06.043

32. A water-sprayable, thermogelating and biocompatible polymer host for use in fluorescent chemical sensing and imaging of oxygen, pH values and temperature

Xu-dong Wang*, R. J. Meier, Carina Schmittlein, Stephan Schreml, Michael Schäferling, O. S. Wolfbeis.

Sens. Actuators B. 2015,221, 37-44.

DOI: 10.1016/j.snb.2015.05.082

31. Optical Methods for Sensing and Imaging of Oxygen

Xu-dong Wang, O. S. Wolfbeis*.

Chem. Soc. Rev. 2014, 43, 3666-3761.

DOI: 10.1039/C4CS00039K

30. A colorimetric agarose gel for formaldehyde measurement based on nanotechnology involving Tollens reaction

Jing-bin Zeng, Shi-guang Fan, Cui-ying Zhao, Qian-ru Wang, Ting-yao Zhou, Xi Chen, Zi-feng Yan, Yan-peng Li, Wei Xing and Xu-dong Wang*.

Chem. Comm. 2014, 50, 8121-8123.

DOI: 10.1039/c4cc00914b

29. A High-Resolution Colorimetric Assay for Rapid Visual Readout of Phosphatase Activity Based on Gold/Silver Core/Shell Nanorod

Zhuangqiang Gao, Kaichao Deng, Xu-dong Wang, Manuel Miró, Guonan Chen, and Dianping Tang*.

ACS Appl. Mater. Interfaces. 2014, 6, 18243-18250.

DOI: 10.1021/am505342r

28. Au@Ag core/shell nanoparticles as colorimetric probes for cyanide sensing

Jing-bin Zeng, Ying-Ying Cao, Jing-Jing Chen, Xu-dong Wang, Jian-Feng Yu, Bin-Bin Yu,Zi-feng Yan, Xi Chen.

Nanoscale, 2014, 6, 9939-9943.

DOI: 10.1039/c4nr02560a

27. Luminescent Sensing and Imaging of Temperature

Xu-dongWang*,O. S. Wolfbeis, R. J. Meier*.

Chem.Soc. Rev. 2013, 42, 7834-7869.

DOI: 10.1039/C3CS60102A

26. Fluorescent pH-Sensitive Nanoparticles in an Agarose Matrix for Imaging of Bacterial Growth and Metabolism

Xu-dong Wang*, Robert J. Meier, andOtto S. Wolfbeis*.

Angew. Chem. Int.Ed. 2013, 52, 406-409.

DOI: 10.1002/anie.201205715

25. Fiber-Optic Chemical Sensors and Biosensors (2008−2012)

Xu-dong Wang*,and O. S. Wolfbeis*. Fiber-optic Chemical andBiosensors.

Anal. Chem. 2013,85, 487-508.

DOI: 10.1021/ac303159b

24. Imaging of cellular oxygen via two-photon excitation of fluorescent sensor nanoparticles

Xu-dong Wang, Daniela E. Achatz,Christina Hupf, Michaela Sperber, Joachim Wegener, Sebastian Bange, John M.Lupton, Otto S. Wolfbeis.*

Sens. Actuators B, 2013, 188, 257-262.

DOI: 10.1016/j.snb.2013.06.087

23. Ultra-small, highly stable, and membrane-impermeable fluorescent nanosensors for oxygen

Xu-dong Wang*, Judith A Stolwijk,Michaela Sperber, Robert Johannes Meier, Joachim Wegener and Otto S Wolfbeis.

Methods Appl. Fluoresc. 2013, 1, 035002.

DOI: 10.1088/2050-6120/1/3/035002

22. Ratiometric luminescence 2D in vivo imaging and monitoring of mouse skin oxygenation

Julian, H.; Robert, J. M.; Alexander, M.; Valentin,S.; Florian, B.; Regina, T.; Christian, B.; Gregor, L.; Xu-dong, Wang; Otto, S. W.; Jonathan, J. Ratiometric luminescence2D in vivo imaging and monitoring of mouse skin oxygenation. Methods Appl. Fluoresc. 2013, 1, 045002.

DOI: 10.1088/2050-6120/1/4/045002

21. Ultra-Small, Highly Stable, and Sensitive Dual Nanosensors for Imaging Intracellular Oxygen and pH in Cytosol

Xu-dong. Wang*, J. A. Stolwijk, T. Lang, M. Sperber, R. J. Meier,J. Wegener, O. S. Wolfbeis*. J. Am. Chem. Soc. 2012, 134, 17011-17014. Highlighted in JACS Spotlights (J. Am. Chem. Soc. 2012, 134, 18151−18152).

DOI: 10.1021/ja308830e

20. A Fluorophore-Doped Polymer Nanomaterial for Referenced Imaging of pH and Temperature with Sub-Micrometer Resolution

Xu-dong Wang*, R. J. Meier, O. S. Wolfbeis*. 

Adv. Funct. Mater. 2012, 22, 4202-4207.

DOI: 10.1002/adfm.201200813

19. Study of oxygen effects on electrochemiluminescence using dye-doped oxygen-resisting nanobeads

Lan Luan, Zhi-jie Lin, Xi-wei Liu, Xu-dongWang, X. Chen,

Analyst 2012, 137, 2459-2461.

DOI: 10.1039/c2an00026a

18. Self-referenced RGB colour imaging of intracellular oxygen

Xu-dong Wang, H. H. Gorris*, J. A. Stolwijk, R. J. Meier,D. B. M. Groegel, J. Wegener, O. S. Wolfbeis*.

Chem. Sci, 2011,2, 901-906.

DOI: 10.1039/c0sc00610f

17. Preparation of Reversible Colorimetric Temperature Nanosensors and Their Application in Quantitative Two-Dimensional Thermo-Imaging

Xu-dong Wang, Chun-yan He, Zhao-xiongXie, Xi Chen*.

Anal. Chem. 2011, 83, 2434-2437.

DOI: 10.1021/ac200196y

16. Chameleon clothes for quantitative oxygen imaging

Xu-dong Wang, Ting-yao Zhou, Xin-hongSong, Yaqi Jiang, Chaoyong James Yang, Xi Chen*.

J. Mat.Chem. 2011, 21,17651-17653. Highlighted in Chemical World和Education in Chemistry.RSC “Hot communication”.

DOI: 10.1039/c1jm14162g

15. Simultaneous Photographing of Oxygen and pH In Vivo Using Sensor Films

R.J. Meier, S. Schreml, Xu-dong Wang,M. Landthaler, P. Babilas, O. S. Wolfbeis.

Angew. Chem.Int. Ed. 2011, 50. 10893-6.

DOI: 10.1002/anie.201104530

14. (Ru(dpp)3( ClO4)2) 掺杂的聚丙烯腈纳米颗粒的制备及其在比率荧光pH检测中的应用

Ting-xiuYe, Xu-dong Wang, Xiao-xia Chen,Ying-xue Zhang, Yan-qin Qu, Xi Chen.

Journal of FuzhouUniversity, 2011, 39, 765-768.

13. 双原色发光比色氧传感器

Ting-yaoZhou, Xu-dong Wang, Xi-wei Liu, Xi Chen.

Scientia Sinica Chimica, 2011, 41, 678-682.

DOI: 10.1360/032011-15

12. Photographing Oxygen Distribution

Xu-dong Wang, Robert J. Meier, LinkMartin, Otto S. Wolfbeis*.

 Angew. Chem. Int. Ed. 2010, 49, 4907-4909.

Highlighted in NewScientist.

DOI: 10.1002/anie.201001305

11. Optical oxygen sensors move towards colorimetric determination

Xu-dong Wang, Hai-xu Chen, Yun Zhao, XiChen*, Xiao-ru Wang.

Trends in Anal. Chem. 2010, 29,319-338.

DOI:10.1016/j.trac.2010.01.004

10. Colorimetric optical pH sensor production using a dual-color system

Hai-xu Chen, Xu-dongWang, Xin-hong Song, Ting-yao Zhou, Ya-qi Jiang, Xi Chen*. Colorimetricoptical pH sensor production using a dual-color system.

Sens. Actuators B 2010, 146, 278-282.

DOI: 10.1016/j.snb.2010.01.068

9. Extended detection range for an optical enzymatic glucose sensor coupling with a novel data-processing method

Guang-mei Guo, Xu-dongWang, Ting-yao Zhou, Xi Chen*.

Science China-Chemistry 2010, 53, 1385-1390.

DOI: 10.1007/s11426-010-3161-6

8. Optical colorimetric sensor strip for direct readout glucose measurement

Xu-dong Wang, Hai-xu Chen, Zhi-jie Lin,Ting-yao Zhou, Jing-bin Zeng, Zhao-xiong Xie, Xi Chen*, and Xiao-ru Wang.

Biosens. Bioelectron. 2009, 24, 3702-3705.

DOI: 10.1016/j.bios.2009.05.018

7. Fabrication of a Colorimetric Electrochemiluminescence Sensor

Zhi-jie Lin, Xiao-mei Chen, Tian-tian Jia, Xu-dong Wang, Zhao-xiong Xie, MunetakaOyama, Xi Chen*. 

Anal. Chem. 2009, 81, 830-833.

DOI: 10.1021/ac8020054

6. Reversible Optical Sensor Strip for Oxygen

Xu-dong Wang, Xi Chen*, Zhao-xiong Xie, andXiao-ru Wang.

Angew. Chem. Int. Ed. 2008, 47, 7450-7453. Highlighted in Nature Materials (2008, 7, 746) and Nature Asia Materials (2008-12-19).

DOI: 10.1002/anie.200801733

5. An optical biosensor for the rapid determination of glucose in human serum

Xu-dong Wang, Ting-Yao Zhou, Xi Chen*,Kwok-Yin Wong, Xiao-Ru Wang.

Sens. Actuators B 2008, 129, 866–873.

DOI: 10.1016/j.snb.2007.09.079

4. Study on the Fluorescence Characteristics of BOD Sensing Films Immobilizing Different Limnetic Microorganism

Guang-mei Guo, Ling-ling Xin, Xu-dong Wang, Yun Zhao, Xi Chen*.

Spectroscopyand Spectral Analysis 2008,28, 2134-2138.

DOI: 10.3964/j.issn.1000-0593(2008)09-2134-05

3. Development of Portable Sensing Apparatus for Biochemical Oxygen Demand Determination based on Fluorescent Response

Guang-mei Guo, Yun Zhao, Yu-hua Weng, Xu-dong Wang, Xi Chen*, Xiao-ru Wang.

 Chinese J. Anal. Chem. 2008,36(4), 563-566.

2. BOD Sensing Film Based on Fluorescence Quenching by Oxygen in Freshwater

Ting-yao Zhou, Xu-dong Wang, Yun Zhao, Hai-Xu Chen, XiChen.

Journal of Xiamen University, 2008,47, 208-212.

1. An optical biosensing film for biochemical oxygen demand determination in seawater with an automatic flow sampling system

Ling-ling Xin, Xu-dongWang, Guang-mei Guo, Xiao-ru Wang and Xi Chen*.

Meas. Sci. Technol. 2007, 18, 2878-2884.

DOI: 10.1088/0957-0233/18/9/017

Nanomaterials for Intracellular pH Sensing and Imaging

Novel Nanomaterials for Biomedical, Environmental, and Energy Applications is a comprehensive study of cutting-edge progress in the synthesis and characterization of novel nanomaterials and the subsequent advances in biomedical, environmental and energy applications. Covering novel concepts and key technical points of interest, this book explores the frontier applications of nanomaterials.

有机改性溶胶-凝胶固定葡萄糖氧化酶的方法

本发明提供一种有机改性溶胶- 凝胶固定葡萄糖氧化酶的方法，将四甲氧基硅烷与二甲基二甲氧基硅烷混匀后加入与四甲基硅烷同体积的HCl 水溶液；将混合液置于小瓶中，加带小孔的盖子于水浴中搅拌水解；静置3 ～ 5 分钟，留下层凝胶液备用；将聚乙烯醇水溶液和制备的凝胶液混合，静置3 ～ 5 分钟后，取静置分层后的上清液与溶解有葡萄糖氧化酶的磷酸缓冲液混合，在所需基质表面均匀涂膜，干燥成型。本发明成功的解决了单纯使用PVA 包埋存在的溶胀性问题；葡萄糖氧化酶被物理地包埋在凝胶液与PVA 杂化形成的多孔基质中，不易发生流失；保持了葡萄糖氧化酶的活性和稳定性，在冰箱中能长期保存

溶胶-凝胶固定水溶性量子点的方法

溶胶- 凝胶固定水溶性量子点的方法，涉及一种半导体纳米粒子。提供一种操作简单，能快速有效地在实现水溶性量子点的固定并保持量子点的发光特性的溶胶- 凝胶固定水溶性量子点的方法。将3- 氨基丙基- 三甲氧基硅烷和甲醇混匀得混合液A ；将CdCl2和巯基乙酸混匀，用NaOH 调节溶液pH 得离子溶液B ；取混合液A 置于容器中加入超纯水，搅拌使3- 氨基丙基- 三甲氧基硅烷水解得混合液C ；将盛有混合液C 的容器封口，在封口膜上穿孔，将盛有混合液B 的容器恒温；取出盛有混合液B 的容器，加入水溶性量子点和离子溶液B 得混合液D ；将盛有混合液C 的容器恒温后，即得固定有水溶性量子点的凝胶膜。

一类可视化氧传感检测的生物传感器的制备方法

一类可视化氧传感检测的生物传感器的制备方法，涉及一种生物传感器。提供一种具有成本低、灵敏度高、分辨率好、准确度高等特点的可视化氧传感检测的生物传感器的制备方法。将四乙氧基硅烷、3- 氨基丙基- 三甲氧基硅烷、超纯水和甲醇混匀，加入绿色量子点溶液和Cd 前驱体溶液，于玻璃片上涂膜得发光稳定的量子点层。将四甲氧基硅烷和二甲基二甲氧基硅烷加入HCl 加热，取下层凝胶液与PtF20TPP 的四氢呋喃溶液混合，涂布于量子点层上成膜。将TMOS 与DiMe-DMOS混匀，加入HCl 水溶液，水解留下层凝胶液；将聚乙烯醇水溶液和凝胶液混合，取上层液体与溶解有微生物或酶的磷酸盐缓冲液混合，于氧传感层表面均匀涂膜成型

一种食品中丙烯酰胺的检测方法

一种食品中丙烯酰胺的检测方法，涉及一种丙烯酰胺，尤其是涉及一种烤、烘、炸、煎类等食品中的AM 含量的荧光快速检测方法。提供一种操作简单、灵敏度高、重现性好、检测快速准确、实用价值高、经济性与可靠性好、易于推广使用的一种食品中丙烯酰胺的检测方法。将研磨成粉的样品放入容器中，加入正己烷，超声脱脂后，吹干残留的正己烷，得产物A ；在产物A 中加入NaCl 溶液，超声萃取，离心后的残渣用NaCl 溶液重复萃取一次，合并萃取液；在萃取液中加入氢氧化钠溶液，再加入次氯酸钠溶液，反应后的混合溶液用磷酸盐缓冲溶液调节混合溶液为碱性，最后加入荧光胺溶液，混匀反应，放入荧光仪中检测。

基于卟啉铂配合物荧光猝灭原理实现氧传感检测的传感膜

本发明提供一种基于卟啉铂配合物荧光猝灭原理实现氧传感检测的传感膜，其制备方法为：将0.1mol/L 的HCl，曲拉通X-100，二次蒸馏水，n- 丙基三甲氧基硅烷，乙醇和3，3，3- 三氟丙基三甲氧基硅烷按体积比1 ∶ 1 ∶ 8 ∶ 8 ～18 ∶ 17 ∶ 15 ～ 19 搅拌混匀。将混合液置于小瓶中，加盖密封盖，于室温下搅拌1 小时；取反应后溶胶- 凝胶液与溶解有中位-( 五氟苯基) 卟啉铂配合物的四氢呋喃溶液按体积比4 ∶ 1 混合，室温下搅拌5 ～ 15 分钟，在所需基质表面均匀涂膜，室温干燥成膜。该传感膜能有效包埋卟啉铂配合物，并具有较好的通透性，可实现环境中氧含量的传感测定。

发明专利-一种可逆比色氧传感布标签的制备方法

一种可逆比色氧传感布标签的制备方法。涉及比色传感器，提供一种成本低、亮度高、灵敏度好、构造简单，可实现氧浓度可视化快速测定的比色氧传感布标签及其制备方法。包括两种荧光染料、聚苯乙烯微球和布，两种荧光染料包裹在聚苯乙烯微球内，其中一种荧光染料对氧敏感，聚苯乙烯微球固定在布上。制备方法包括：制备含有两种荧光染料的聚苯乙烯微球，并将其固定在布上，即得可逆比色氧传感布标签。本发明制备的比色氧传感布标签，在使用时只需要用一个手持式紫外发光二极管进行照射，比色氧传感布标签会发出非常明亮的色光。蓝色荧光染料和对氧敏感红色荧光染料都可以被紫外光激发，可实现可视化直接检测，不需要任何滤光片等光学设备