In this work, we have observed that OSPs-doped solid silica nanoparticles show good response to molecular oxygen. They not only show good sensitivity and fast response to oxygen but also exhibit excellent size and morphology control and good biocompatibility. Interestingly, oxygen-sensing performance can be tuned by changing the types of OSPs and silica morphologies. Because of their “clean” outer surface, these nanosensors can be further functionalized with arbitrary units for intracellular active-targeting and multiple purposes of applications. We have demonstrated the ease of functionalization by modifying the surface of nanosensors with organelle-targeting groups and used them for sensing oxygen concentration at subcellular level. Owing to the long luminescent lifetime of the nanosensors, we are able to image intracellular oxygen concentration using our custom-built microscopic rapid lifetime imaging system. The combination of rapid lifetime imaging technique with solid-silica based nanosensors shows superior accuracy and reproducibility, which provides important tools for intracellular oxygen studies, and allows us quantitatively imaging and tracing intracellular oxygen concentration over time and in situ with millisecond time resolution.
READ MOREIn this work, we report quadruple-fluorophore-labeled serum albumins as triple nanosensors for simultaneously imaging intracellular pH, oxygen and temperature. The nanosensors exhibit good biocompatibility and are biodegradable, which endows them suitable for intracellular studies without severely interrupting normal cellular activities. The triple nanosensors are constructed using serum albumins as building blocks. Native bovine serum albumins were modified with amino groups to increase the number of amino groups on protein surface. The reactive amino groups on protein surface were then covalently labeled with four fluorophores, which forms he triple nanosensors. Fluorophores were carefully selected to prevent self-quenching, and signal cross-talking. All three parameters were measured in referenced mode, which greatly enhances the precision and accuracy of intracellular measurements. The influences of cellular autofluorescence and system alignment have been greatly suppressed. Results show the nanosensors have good responses to dissolved oxygen, pH and temperature in the physiological range, which is beneficial for intracellular studies. These nanosensors have been proved to be biodegradable, and can be easily taken up by cells via endocytosis. The triple nanosensors can be directly used for sensing and imaging intracellular oxygen, pH and temperature values.
Prof. Dr. Xu-dong Wang was invited to participate the 14th SCCS conference hold in Datong, China. He gave an invited talk on oxygen sensor based on fluorescence lifetime.
READ MORELongjiang Ding was selected in the "Scientific promotion programs for excellent PhD students". Congratulations!
READ MOREWei Zhang passed the dissertation defense and received her M.S. degrees. Congratulations!
READ MOREThis award honors the outstanding performance of top 5 graduate students.
READ MOREIn this work, we have developed a highly-sensitive dissolved oxygen sensor with excellent mechanical stability and self-cleaning capability. The sensor composes of a micro/nano hierarchical structured superhydrophobic coating that is doped with oxygen-sensitive probe. The hierarchical structure was then solidified using silicone resin to endow the sensor film with good mechanical stability. After tape-peeling, intensive scratching and sandpaper abrasion, the sensor film still maintains good superhydrophobic nature with contact angle larger than 150 o. Owing to the porous structure and high gas permeability of silicone resin, the sensor showed excellent quenchability, toward oxygen quenching with I0/I100 over 77. Because of its superhydrophobic property, algae, biofilm and clams are difficult to adhere on the surface of the sensing film. This feature keeps the sensor surface clean over long-term usage, and there is no need to install cleaning brush, which will make sensor design much simple, more energy-saving and with largely-extended service life. Moreover, the fabrication of the sensor film is very simple, and there is no sophisticated synthesis and fabrication technique involved, which could be applied for massive production.
READ MOREProf. Dr. Xu-dong Wang attended the 2018 China-Japan-Korea Symposium on Analytical Chemistry (CJK 2018) hold in Zhangzhou, China. He gave an invited talk on high performance oxygen nanosensors based on luminophore-doped solid silica nanoparticles.
READ MOREThis award honors the academic excellence and outstanding performance of top 5 postgraduate students.
READ MOREThis award honors the academic excellence and outstanding performance of top 5 PhD students.
READ MOREThis award honors the academic excellence and outstanding performance of top 5 postgraduate students.
READ MOREIn this work, a lysosome-targeting nanosensor has been designed for simultaneous imaging of pH values and temperature in HeLa cells. The dual nanosensor responds to pH values in the range from 3.0 to 9.0, and to temperature in the range from 20 to 60 °C. Owing to its good biocompatibility and good sensitivity, the dual nanosensor was used to monitor changes in local pH values and temperature in the lysosome of HeLa cells. The nanosensors could be easily prepared and the surface functionalization technology is already well established. The remaining surface functional groups on nanosensors can be further explored for cellular active-targeting, carrying drugs, imaging, and tracking purposes.
袁荃，教授，博士生导师，2004年本科毕业于武汉大学，2009年在北京大学获得无机化学博士学位。2009-2011年在美国佛罗里达大学从事博士后研究工作。2011年12月进入武汉大学化学学院工作，2017年9月加入湖南大学化学化工学院。
READ MORE夏帆，教授，博士生导师。华中科技大学本科（应用化学和科技英语双学位），中国科学院化学研究所博士，曾在美国加州大学圣塔芭芭拉分校从事博士后研究工作（导师：艾伦黑格尔院士，诺贝尔化学奖得主）。现任中国地质大学（武汉）材料与化学学院院长，纳米矿物材料及应用教育部工程研究中心主任。
In this work, we are introducing a new background-subtraction strategy, which has been proved to be a powerful tool to standardize the sensor response. By subtracting the scattered background light at a long wavelength, every oxygen sensor film exhibits an identical calibration curve and sensitivity, regardless of the physiochemical properties of the sensor film and instrumental setups. This strategy allows us to develop simple oxygen sensor devices with much lower cost, and the sensor can be calibrated after production. During their application, there is no need to recalibrate them, which is beneficial for their applications in industry, especially for long-term monitoring of oxygen concentration.
READ MORE何华瑞博士，南开大学硕士，奥地利格拉兹大学博士（Otto S Wolfbeis课题组），天津希恩思生化科技有限公司董事长兼技术总监。天津市“千人计划”创业人才、 高新区海外高层次人才。研究方向为便携式现场快速检测仪、试剂盒以及相关临床诊断试剂、化学及生物传感器及其在临床诊断中的应用和免疫诊断法。
Hans H. Gorris received his Ph. D degree in University of Lübeck (Germany) in 2005. In 2006-2009 he worked as a postdoctoral fellow at the Department of Chemistry, Tufts University, Medford (USA). Since 2009 he is an assistant Professor at the Institute of Analytical Chemistry, Chemo- und Biosensors, University of Regensburg (Germany). His research interests are bioanalytical assays, fluorescence microscopy, photon-upconversion nanoparticles (UCNPs), single molecule enzymology, single molecule immunoassays.
Intracellular pH is a vital parameter that precisely controls cell functionalities, activities and cellular events. Abnormal intracellular pH is always closely related to the healthy status of cells, which is further translated into pathological changes in a macro perspective. In this chapter, we will start from illustrating the importance of measuring intracellular pH, and further discuss how to design optical nanosensors for sensing and imaging intracellular pH. The state of the art technology in intracellular pH sensing and imaging will be reviewed, nanomaterials that are used for constructing intracellular pH sensors will be summarized and the perspective of nanomaterials for intracellular pH sensing and imaging will be given at the end.
READ MOREProf. Dr. Xu-dong Wang was invited to participate the 13th CCS National Conference on Analytical Chemistr hold in Xi'an, China. He gave an invited talk on optical multi-parameter nanosensors and their intracellular fluorescent applications.
READ MOREIn this work, we have developed a highly sensitive, fully reversible, and reliable optical sensor for H2O2, with fast response and excellent stability. Highly sensitive oxygen sensor film was integrated with an ultrastable and highly efficient catalytical layer to form a sensor film, which can rapidly convert H2O2 into oxygen. The concentration of decomposed oxygen was sensitively measured by a luminescence-quenching-based oxygen sensor. Owing to our in situ growth strategy and the rational-designed nanostructure, the sensor exhibits extremely high stability over long time without observed loss of performance. The specially designed fibrous nanostructure also largely enriched catalyst concentration in the sensor film, which ensures the sensor having fast response (<1 min), good stability, and full reversibility. Because of the simplicity of the sensor design, the new H2O2 sensor is fully compatible with all commercial-available optical oxygen sensor devices, which makes the application of such sensors much easier and more economically friendly.
Prof.Dr. Xu-dong Wang attended the 31th annual conference of the Chinese Chemical Association and give a talk on Optical Oxygen Sensor.
Prof. Dr. Xu-dong Wang was invited to participate the 31th annual conference of the Chinese Chemical Association hold in Hangzhou, China. He gave an invited talk on a background-substraction strategy to calibrate optical oxygen sensor for nearly identical response, in which he presented the lastest research in our group on optical sensor for oxygen sensor.
Torsten Mayr received his PhD in chemistry from the University of Regensburg (Germany) in 2004. In 2002–2004 he was a post-doctoral fellow at the Karolinska Institute in Stockholm (Sweden). Since 2004 he is Assistant Professor at the Institute of Analytical Chemistry and Food Chemistry at the Graz University of Technology. Since 2014 he is Associate Professor the same institute. His research dedicated to optical chemical sensors and their application in biotechnology and environmental analyses. Further research activities include functionalized micro- and nanoparticles, luminescent materials and the integration of sensors in microfluidic systems. Since 2017 he is member of the editorial advisory board of the Journal “Analytical Chemistry” of the ACS.
Europt(r)ode is a non-for-profit organization with world coverage (yet of European origin) devoted to stimulate and spread the interest of the scientific and technical community, as well as the general public, for the research, development and applications for the welfare of society, of optical chemical sensors and biosensors.
It is one of the leading organizations in optical sensors, the Eutroptrode conference is held in every two years and the conference has a unique permanent steering commitee to make sure the high quality of the conference. In the latest commitee meeting in Naples, Prof. Wang was nominated as one of the member for the Permanent Steering Commitee.
For more information, please visit http://www.europtrode.org/
READ MORELian Ying is a Ph.D student in her first year study, and has published one paper in just half of a year research. Congratulations!
In this work, we have described the synthesis of Cyanine dye doped silica nanoparticles. Cyanine dyes has been widely used in biological imaging since its attractive features of long emission wavelength. However, the dye is not very stable and tends to degrade in harsh conditions. We have supperisedly observed that most of cyanine dyes cannot be encapsulated via water-in-oil emulsion approach to form dye-doped silica nanoparticles, which is out of expectation. However, Cy-doped silica particle can be easily synthesized via Stober apporach which has good morphology, size distribution and Brightness.
The Europt(r)ode is one of the leading worldwide conference for the presentation of innovations in chemical e biochemical optical sensing, showing both cutting-edge integration concepts and key emerging trends in a highly multi-disciplinary atmosphere. Since 1992, the EUROPT(R)ODE conference attracts scientists and engineers from academia, research institutes and industry from all over the world on all aspects related to the research, development and applications of optical chemical sensors and biosensors
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